Chemical Synthesis of a Locked Nucleic Acid–Substituted Dinucleotide Cap Analog
نویسندگان
چکیده
منابع مشابه
Synthesis and biological properties of triazole-linked locked nucleic acid.
We have synthesized and studied the biological and biophysical properties of triazole-linked ribo and xylo locked nucleic acid (LNA). The combination of LNA with the Isobe triazole linkage gave high binding affinity when incorporated at the 3' or 5' termini of oligonucleotides, but low binding affinity at internal positions. Antisense oligonucleotides (ASOs) and siRNAs containing triazole dimer...
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Modified nucleic acids have considerable potential in nanobiotechnology for the development of nanomedicines and new materials. Locked nucleic acid (LNA) is one of the most prominent nucleic acid analogues reported so far and we herein for the first time report the enzymatic incorporation of LNA-U and C5-ethynyl LNA-U nucleotides into oligonucleotides. Phusion High Fidelity and KOD DNA polymera...
متن کاملLocked Nucleic Acids (LNAs)
With few exceptions, experimental success in molecular biology is dependent upon specific, discriminating, and persistent hybridization events involving synthetic oligonucleotides and their complementary target sequences. While unmodified oligodeoxynucleotides will routinely form desired DNA:DNA and DNA:RNA duplexes, synthesis of various modifications that confer enhanced high-affinity recognit...
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A novel LNA-MB (molecular beacon based on locked nucleic acid bases) has been designed and investigated. It exhibits very high melting temperature and is thermally stable, shows superior single base mismatch discrimination capability, and is stable against digestion by nuclease and has no binding with single-stranded DNA binding proteins. The LNA-MB will be widely useful in a variety of areas, ...
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Adenosine analogs in which the sugar unit was replaced by a 1,4-dioxane sugar equivalent, were prepared by coupling the 1,4-dioxane sugar analog as its anomeric acetates, with N6-benzoyl protected adenine. The 1,4-dioxane system was obtained in an enantioselective synthesis from (R,R)-dimethyl tartrate. Using standard phosphorimidite methodology, the adenine analog was further reacted with a 1,...
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ژورنال
عنوان ژورنال: Current Protocols
سال: 2021
ISSN: 2691-1299,2691-1299
DOI: 10.1002/cpz1.22